Zeiss LSM 510 META Especificaciones Pagina 85
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OPERATION
LSM 510 Image Acquisition (Acquire)
B 40-051 e 07/98 4-27
4.5 Image Acquisition (Acquire)
In the main menu toolbar, click on <Acquire>.
This opens another, subordinate toolbar in the main menu.
Fig. 4-30
☞
For preparing and acquiring a scanning image, it is recommended to call up and use the tools
of the subordinate toolbar in succession from left to right.
Laser setting.
Conventional microscope setting.
Configuring the optical system for the Scanning Mode.
Setting of scan parameters.
<EditROI> permits up to 10 areas within a frame to be defined and scanned.
<TimeSeries> permits user-specific time series to be selected for the scan procedure.
The <EditBleach> function is used to bleach a defined, freely selectable area within the scanning field.
Upon selecting <Stage> you can set the focus (Z coordinate) and the Z step size between successive
slices. If the optional, motorized X/Y-stage is connected, the X and Y-positions of the sample can also
be selected.
With the <VIS>, <TV> and <LSM> buttons you can effect a PC-supported switching of the beam
path.
VIS for conventional microscopy through the eyepieces.
TV conventional microscopy for observation by means of a TV camera.
LSM Laser scanning mode.
☞
For the scanning process, the <LSM> button in the toolbar subordinate to the "Acquire" item
must be activated, and the silver slider on the microscope must be in the LSM position.
Toolbar subordinate to the
<Acquire> item
- Laser Scanning Microscope 1
- B 40-051 e 07/98 III 3
- IV B 40-051 e 07/98 4
- Scope of Equipment Supplied 6
- INTRODUCTION 10
- X B 40-051 e 07/98 10
- CONTENTS 11
- NOTES ON DEVICE SAFETY 12
- Contents LSM 510 12
- 1-2 B 40-051 e 07/98 12
- 1 NOTES ON DEVICE SAFETY 13
- Exit aperture 18
- B 40-051 e 07/98 1-9 19
- 1-10 B 40-051 e 07/98 20
- LGK 7774 21
- LGK 7628-1 21
- 1-12 B 40-051 e 07/98 22
- LSM 510 - SETUP REQUIREMENTS 24
- 2-2 B 40-051 e 07/98 24
- Fig. 2-1 25
- Fig. 2-2 25
- Fig. 2-3 26
- Fig. 2-4 27
- 2-12 B 40-051 e 07/98 34
- CHAPTER 3 INTRODUCTION TO 35
- LASER SCANNING MICROSCOPY 35
- 3-2 B 40-051 e 07/98 36
- Module VIS 40
- Scan ModuleMicroscope 40
- Module UV 40
- 3-12 B 40-051 e 07/98 46
- CHAPTER 4 OPERATION 59
- 4 OPERATION 61
- Fig. 4-1 62
- Fig. 4-2 63
- Fig. 4-3 63
- Fig. 4-4 64
- Fig. 4-5 65
- Fig. 4-6 66
- Fig. 4-7 66
- Fig. 4-8 67
- Fig. 4-9 67
- Fig. 4-10 68
- Fig. 4-11 69
- Fig. 4-12 70
- Fig. 4-13 71
- Fig. 4-14 72
- Fig. 4-15 73
- Fig. 4-16 74
- Fig. 4-17 75
- Fig. 4-18 76
- Fig. 4-19 77
- Fig. 4-20 78
- Fig. 4-21 79
- Fig. 4-22 80
- Fig. 4-23 82
- Fig. 4-24 82
- Fig. 4-25 83
- Fig. 4-26 83
- Fig. 4-27 83
- Fig. 4-28 84
- Fig. 4-29 84
- Fig. 4-30 85
- Fig. 4-31 86
- Fig. 4-32 88
- Fig. 4-33 89
- Fig. 4-34 90
- Fig. 4-35 90
- Fig. 4-36 91
- Fig. 4-37 92
- Fig. 4-38 93
- Fig. 4-39 95
- Fig. 4-40 96
- Fig. 4-41 97
- Fig. 4-42 98
- Fig. 4-43 100
- Fig. 4-44 101
- Fig. 4-45 103
- OPERATION 104
- 4-46 B 40-51 e 07/98 104
- B 40-051 e 07/98 4-47 105
- Fig. 4-48 106
- Fig. 4-49 107
- Fig. 4-50 108
- Fig. 4-51 109
- Fig. 4-52 110
- Fig. 4-53 111
- Fig. 4-54 112
- Fig. 4-55 113
- Fig. 4-56 114
- Fig. 4-57 115
- Fig. 4-58 115
- Fig. 4-59 116
- Fig. 4-60 117
- Fig. 4-61 118
- Fig. 4-62 119
- Fig. 4-63 120
- Fig. 4-64 121
- Fig. 4-65 122
- Fig. 4-66 123
- B 40-051 e 07/98 4-67 125
- Fig. 4-68 126
- Colored marker positions 126
- Fig. 4-69 127
- Fig. 4-70 128
- Fig. 4-71 129
- Fig. 4-72 129
- Fig. 4-73 130
- Fig. 4-74 130
- Fig. 4-75 131
- Fig. 4-76 131
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- Fig. 4-78 133
- Fig. 4-79 133
- Fig. 4-81 134
- Fig. 4-80 134
- Fig. 4-82 135
- Fig. 4-83 135
- Fig. 4-90 139
- Fig. 4-91 140
- LSM 510 Image Optimization 141
- B 40-051 e 07/98 4-83 141
- Fig. 4-93 142
- Fig. 4-94 143
- Fig. 4-95 144
- B 40-051 e 07/98 4-87 145
- Fig. 4-97 146
- Fig. 4-98 147
- Fig. 4-99 148
- Fig. 4-100 149
- Fig. 4-101 150
- Fig. 4-102 151
- Fig. 4-103 152
- Fig. 4-104 153
- / Ampl. Gain 154
- Fig. 4-105 155
- Fig. 4-106 156
- Fig. 4-107 157
- Fig. 4-108 158
- Fig. 4-109 159
- Fig. 4-110 160
- Fig. 4-111 161
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- Fig. 4-115 164
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- Fig. 4-118 168
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- Fig. 4-120 170
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- 4-124 B 40-051 e 07/98 182
- Fig. 4-133 183
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- Fig. 4-138 188
- Fig. 4-139 189
- Fig. 4-140 190
- Fig. 4-141 191
- 4-134 B 40-051 e 07/98 192
- Fig. 4-143 193
- Fig. 4-144 194
- B 40-051 e 07/98 4-137 195
- Fig. 4-146 196
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- Fig. 4-165 212
- Fig. 4-166 214
- Fig. 4-167 215
- Fig. 4-168 216
- Fig. 4-169 217
- LSM 510 Annex 219
- B 40-051 e 07/98 4-161 219
- Annex LSM 510 220
- 4-162 B 40-051 e 07/98 220
- B 40-051 e 07/98 4-163 221
- 4-164 B 40-051 e 07/98 222
- B 40-051 e 07/98 4-165 223
- 4-166 B 40-051 e 07/98 224
- B 40-051 e 07/98 4-167 225
- 4-168 B 40-051 e 07/98 226
- B 40-051 e 07/98 4-169 227
- 4-170 B 40-051 e 07/98 228
- Fig. 4-170 Filter change 229
- Allen key 230
- B 40-051 e 07/98 4-173 231
- Fine focusing 233
- Fig. 4-173 234
- Fig. 4-174 237
- Fig. 4-175 240
- Fig. 4-176 246
- Fig. 4-177 247
- Fig. 4-178 249
- Fig. 4-179 250
- Fig. 4-180 252
- Fig. 4-181 253
- CHAPTER 5 3D FOR LSM 510 255
- 5-2 B 40-051 e 07/98 256
- Positive rotation directions 257
- Fig. 5-1 257
- Fig. 5-2 259
- Display window 260
- Fig. 5-4 265
- Fig. 5-5 267
- Fig. 5-6 268
- Fig. 5-7 270
- Fig. 5-9 274
- Fig. 5-10 275
- Fig. 5-11 276
- Fig. 5-12 278
- Fig. 5-13 280
- Fig. 5-14 283
- Fig. 5-15 285
- Fig. 5-16 287
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- Fig. 5-18 291
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- Fig. 5-20 292
- Fig. 5-21 293
- Fig. 5-22 295
- Fig. 5-23 297
- Fig. 5-24 299
- Fig. 5-25 301
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- Fig. 5-27 304
- Fig. 5-28 305
- Fig. 5-29 306
- Fig. 5-30 307
- Fig. 5-31 308
- Fig. 5-32 309
- Fig. 5-33 310
- Fig. 5-34 314
- Functions LSM 510 318
- 5-64 B 40-051 e 07/98 318
- Measurement Concept 318
- Fig. 5-36 319
- Fig. 5-37 320
- Fig. 5-39 327
- Measured 329
- Object Features 329
- Volume Features 329
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