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INTRODUCTION TO LASER SCANNING MICROSCOPY
Functions LSM 510
5-64 B 40-051 e 07/98
5.3.5 Functions in the Measurement Menu
Measurement Concept
Measurement is based on regions (objects) in three-dimensional space. Segmenting an image sequence generates
these. The image segmentation process produces a mask image that defines the region.
A region is a group of voxels that touch at the surfaces or at the edges, but not at the corners (18 voxel
neighborhood).
This is illustrated by the following example. The voxels marked black in sequential image Z-1, Z, Z+1 all belong to the
same region as the grey central voxel in sequential image Z. The volume view shows the neighborhood
interrelationships as a 3D projection.
Sequential image: Volume view:
Z-1
Z
Z+1
Fig. 5-35
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