Zeiss LSM 510 META Especificaciones Pagina 281
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INTRODUCTION TO LASER SCANNING MICROSCOPY
LSM 510 Functions
B 40-051 e 07/98 5-27
A grey value range of the Input image sequence is scaled to another range in the Output image
sequence. Both ranges can be edited interactively. This function is used to achieve a better view of an
image sequence, or to scale a range of grey values to single value for a special coding in an image
sequence. The function does not improve the result of the linear segmentation function Segment.
Input indicates the sequence to enhance. If it is a multichannel sequence, a single channel, all channels,
or any number can be selected. The Input histogram shows the grey value distribution of the selected
channels of the Input image sequence.
Output defines the name of the result sequence. It will get only those channels which are chosen by the
Input parameter. The buttons labeled with 8 and 12 define the grey value (intensity) resolution in bit.
Normally the result will get the same resolution as the Input sequence. A change will be needed if image
sequences with different resolutions should be combined. Rising the grey value range to 12 bit will not
enhance the display quality or measurement accuracy. The smooth and morphology functions will
produce results with finer gradations.
If Clip Grey Values is selected, the output grey values are clipped to the Low (L) and High (H) values. If
Clip Grey Values is not selected, output grey values beyond the Low and High value range are
possible.
The Output histogram shows the resulting histogram. The horizontal axis represents the grey values
from 0 to the maximum, which is either 255 or 4095, depending whether the input is 8 bit or 12 bit.
The vertical axis represents the pixel count. The selected range is marked by the borderlines in the
histogram. The blue line or L indicates the lower boundary, the red line or H the upper one, C indicates
the center of the range.
There are three ways to change the range: clicking and dragging the borderlines with the mouse.
- Laser Scanning Microscope 1
- B 40-051 e 07/98 III 3
- IV B 40-051 e 07/98 4
- Scope of Equipment Supplied 6
- INTRODUCTION 10
- X B 40-051 e 07/98 10
- CONTENTS 11
- NOTES ON DEVICE SAFETY 12
- Contents LSM 510 12
- 1-2 B 40-051 e 07/98 12
- 1 NOTES ON DEVICE SAFETY 13
- Exit aperture 18
- B 40-051 e 07/98 1-9 19
- 1-10 B 40-051 e 07/98 20
- LGK 7774 21
- LGK 7628-1 21
- 1-12 B 40-051 e 07/98 22
- LSM 510 - SETUP REQUIREMENTS 24
- 2-2 B 40-051 e 07/98 24
- Fig. 2-1 25
- Fig. 2-2 25
- Fig. 2-3 26
- Fig. 2-4 27
- 2-12 B 40-051 e 07/98 34
- CHAPTER 3 INTRODUCTION TO 35
- LASER SCANNING MICROSCOPY 35
- 3-2 B 40-051 e 07/98 36
- Module VIS 40
- Scan ModuleMicroscope 40
- Module UV 40
- 3-12 B 40-051 e 07/98 46
- CHAPTER 4 OPERATION 59
- 4 OPERATION 61
- Fig. 4-1 62
- Fig. 4-2 63
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- OPERATION 104
- 4-46 B 40-51 e 07/98 104
- B 40-051 e 07/98 4-47 105
- Fig. 4-48 106
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- B 40-051 e 07/98 4-67 125
- Fig. 4-68 126
- Colored marker positions 126
- Fig. 4-69 127
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- Fig. 4-90 139
- Fig. 4-91 140
- LSM 510 Image Optimization 141
- B 40-051 e 07/98 4-83 141
- Fig. 4-93 142
- Fig. 4-94 143
- Fig. 4-95 144
- B 40-051 e 07/98 4-87 145
- Fig. 4-97 146
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- / Ampl. Gain 154
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- LSM 510 Annex 219
- B 40-051 e 07/98 4-161 219
- Annex LSM 510 220
- 4-162 B 40-051 e 07/98 220
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- B 40-051 e 07/98 4-169 227
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- Fig. 4-170 Filter change 229
- Allen key 230
- B 40-051 e 07/98 4-173 231
- Fine focusing 233
- Fig. 4-173 234
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- Fig. 4-176 246
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- Fig. 4-179 250
- Fig. 4-180 252
- Fig. 4-181 253
- CHAPTER 5 3D FOR LSM 510 255
- 5-2 B 40-051 e 07/98 256
- Positive rotation directions 257
- Fig. 5-1 257
- Fig. 5-2 259
- Display window 260
- Fig. 5-4 265
- Fig. 5-5 267
- Fig. 5-6 268
- Fig. 5-7 270
- Fig. 5-9 274
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- Fig. 5-33 310
- Fig. 5-34 314
- Functions LSM 510 318
- 5-64 B 40-051 e 07/98 318
- Measurement Concept 318
- Fig. 5-36 319
- Fig. 5-37 320
- Fig. 5-39 327
- Measured 329
- Object Features 329
- Volume Features 329
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