
INTRODUCTION TO LASER SCANNING MICROSCOPY
LSM 510 Functions
B 40-051 e 07/98 5-57
Method
The Input sequence defines the data to be reconstructed. If it is a multichannel sequence one or all
channels can be selected for the reconstruction.
Output sets the name of the result image (sequence). If the sequence exists it is overwritten. Pressing
the button New will generate a new name (number). The size of the sequential images in Output is
determined by the size of the sequential images in Input.
Number of Views determines the number of reconstructions which should be computed. The radio
buttons Start and End define which angle settings are currently shown. A definition for the angle End is
only necessary if Number of Views is higher than 1. If this is true the result sequence will get views
from the Start to the End angle definition. The other reconstructions are determined through the
linearly interpolated intermediate angles. The direction of view is determined from the angles as follows:
The angle Angle Z determines the rotation of the direction of view on the Z-axis. The angle Angle Y
determines the rotation of the direction of view on the Y-axis that has been rotated by the angle
Angle Z. The angle Angle X determines the rotation of the direction of view on an X-axis that is rotated
by Angle Z and Angle Y.
Channel defines if the following parameters are valid for All or just for one. Defining the thresholds for
the channels independently is useful if the grey value boundaries of the objects differ too much in the
different channels. The thresholds Grey Low and Grey High define the grey value range of the objects.
The parameter Aperture is a measure of the size of the highlights. Small values generate large
highlights. Large values generate small highlights (similar to a spot).
Comentarios a estos manuales